AKT1 active Human, recombinant, N-terminal His tag

Size: 10 µg

Description: Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal HIS6 tag, MW=60.2 kDa, co-expressed with PDK1 in an baculovirus infected Sf9 cell expression system. Specific Activity: 12.32 pmol/min/μg enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour.Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Formulated in:25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT. Stability:>6 months at –80°C References: 1. Kuijl C, et al., Nature. 2007 Nov 29;450 (7170):725-30. 2. Vogiatzi P and Giordano A. Cancer Biol Ther. 2007 Aug 3;6(10).