商品介绍
DescriptionLeukemia Inhibitory Factor (LIF), a lymphoid factor, belongs to the interleukin 6 class cytokine1. LIF binds to the specific LIF receptor (LIFR-α) and forms a heterodimer with the GP130 signal transducing subunit, which leads to the activation of the JAK/STAT and MAPK cascades2. LIF is also involved in a wide variety of biological processes including hematopoietic cell proliferation, neuronal survival and formation, and bone formation. It is used to maintain the pluripotency of mouse embryonic stem cell (ESC). Using the current human ESC culture method, human LIF is not required. But recent studies showed that the human ESC is the prime stem cell. The more totipotent human ESC, or called naïve stem cell, which is more like mouse ESC, can be isolated using LIF or inducedby introducing Oct4, Sox2, Klf4, cMyc, with small molecules3,4 or plus two other factors Nr5a2, and RxRγ into the differentiated cells5.
.
Purity > 99% (by SDS Page)
Buffer Phosphate buffered saline with 1% w/v BSA.
Endotoxin Level < 1.0 EU/mg as determined by the LAL method
Biologic Activity The specific activity is at least 1 x 106 units/ml, where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 myeloid leukemia cell colonies in 1 ml agar cultures.
Storage & HandlingStore at 4°C.Freeze-thaw should be avoided.
Stability Stable for up to 6 months from date of receipt when stored as directed.
References 1.Williams R.L., Hilton, D.J., Pease, S., Willson, T.A., Stewart, C.L., et al (1988)
Myeloid leukemia inhibitory factor maintains the developmental potential of
embryonic stem cells. Nature 336:686-7.
2. Marina Trouillas, Claire Saucourt, Bertrand Guillotin, Xavier Gauthereau, Jean-Luc Taupin, Jean-François Moreau, Hélène Bœuf (2009) European Cytokine Network 20:51-62.
3. Hanna, J., Cheng, A.W., Saha, K., Kim, J., Lengner, C.J., Soldner, F., Cassady, J.P., Muffat, J., Carey, B.W., and Jaenisch, R. (2010) Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs. Proc. Natl. Acad. Sci. USA 107: 9222-9227.
4. Xu Y, Zhu X, Hahm HS, Wei W, Hao E, Hayek A, Ding S (2010) Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. PNAS 107:8129-34.
5. Wang W, Yang J, Liu H, Lu D, Chen X, Zenonos Z, Campos LS, Rad R, Guo G, Zhang S, Bradley A, Liu P. (2011) Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog 1. PNAS 108:18283-8.
Biopioneer Brand:
品牌介绍
BioPioneer Inc专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
公司介绍
产品分类
销售排行
问答列表
暂无问答
厂家直采
全球直采 正品优价
正品保障
厂家直发 有线跟踪
正规清关
CIF100%正规报关，提供发票
及时交付
限时必达 不达必赔
总部地址：
江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125)
工作时间：周一至周五9:00~18:00
技术支持：
短信：18915418616
服务热线：
4000-520-616（全国）
☏ 0512-67156496（苏州）
版权信息：
© 2015- 苏州蚂蚁淘生物科技有限公司 版权所有 中国
友情链接：
免责声明：
本站有部分内容来自互联网，如无意中侵犯了某个媒体 、公司 、企业或个人等的知识产权，请来电或致函告之，
本网站将在规定时间内给予删除等相关处理。
本网站将在规定时间内给予删除等相关处理。
扫描二维码关注官方微信
获取最新促销及产品信息