AKT2 Inactivehuman, recombinant, , N-terminal His tag

Size: 100 µg

Description: Recombinant full-lengthhuman AKT2 was expressed by baculovirusinfection in Sf9 insect cells and thenactivated by GST-PDK1 in vitro. The geneaccession number is NM_001626.

Specific Activity: 5.9 pmol/min/μg. Theenzyme reaction is conducted in a buffercontaining 50 mM HEPES (pH7.5), 10 mMMgCl2, 1 mM EGTA, 200 μM ATP, 0.01%Brij-35, and 2 μM substrate (SER/THR 6,Invitrogen) at room temperature for 1 hour.

Application: Useful for the study ofenzyme kinetics, screening inhibitors, andselectivity profiling.

Fomulated in: 25 mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, and3 mM DTT.

Stability: >6 months at –80°C

References:1. Alessi et al., Current Biology 8: 69, 19982. Cohen et al., FEBS Lett. 410: 3, 1997