Rabbit polyclonal IgG purified by Protein A affinity chromatography

Immunogen: Recombinant full-length human SPARC expressed in human cells

Specificity: Human, mouse, rat (not tested for other species)

Application: Western blot 2-10 ug/mL,ELISA 0.1-1 Ug/mL.

Not tested for other applications.

Preservative: None

Sterility: 0.2 um membrane-filtered and packaged aseptically.

QC tests: SDS-PAGE, Western blotting, Direct ELISA

Formulation: Lyophilized from a sterile filtered solution of phosphate-buffered saline (PBS)

Reconstitution: Add sterile distilled water to a concentration of 1 mg/mL. Further dilutions should be made in appropriate buffers.

Storage and stability: Upon receiving, store the product at -20°C. After reconstitution, store the working aliquots at 2-8°C for up to 2 weeks. For extended storage, aliquot the solution and freeze at -70°C or -20°C. Avoid repeated freezing and thawing.

About SPARC: SPARC (secreted protein, acidic and rich in cysteine) is a matrix associated protein that elicits changes in cell shape, inhibits cell cycle progression, and influences the synthesis of extracellular matrix (ECM). It is also known as osteonectin (ON) or basement-membrane protein 40 (BM-40). SPARC contains two calcium binding sites; an N-terminal acidic region and a C-terminal extracellular calcium binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis.